Scientific Publications

SGS M-Scans and its Senior Scientists (names in green) have established an excellent reputation for major contributions to the research and technical literature, often involving collaborations with Universities and Research Institutes worldwide. Recent publications involving SGS M-Scan include (in reverse chronological order):

Ludwig B, Canarelli S, Reason AJ
A Marriage of Convenience
Ultra high performance chromatography coupled with tandem-mass spectrometry has helped to quantify peptides in biological media.
European Pharmaceutical Contractor Winter 2007

Greer FM, Easton RL
Glycosylated Bioproducts - Breaking Down the Benefits
European BioPharmaceutical Review Winter 2006

van Endert P, Hassainya Y, Lindo V , Bach JM, Blancou P,  Lemonnier F, Mallone R.
HLA class I epitope discovery in type 1 diabetes.
Ann N Y Acad Sci. 2006 Oct;1079:190-7

Saveanu L, Carroll O, Lindo V , Del Val M, Lopez D, Lepelletier Y, Greer F , Schomburg L, Fruci D, Niedermann G, van Endert PM
Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum
Nature Immunology, 2005; 6: 689-697

Hassainya Y,, Kratzer R, Pons-Garcia F, Lindo V , Greer F , Lemonnier F, Niedermann G, van Endert PM:
Identification of naturally processed HLA-A2 restricted proinsulin epitopes by reverse immunology
Diabetes, 2005; 54: 2053-2059

Hensmann M, Li C, Moss C, Lindo V , Greer F , Watts C, Ogun SA, Holder AA, Langhorne J.
Disulfide bonds in merozoite surface protein 1 of the malaria parasite impede efficient antigen processing and affect the in vivo antibody response.
Eur J Immunol. 34(3):639-48 (2004) ; Erratum in: Eur J Immunol. 34(3):908 (2004)

Greer FM.
Biopharmaceutical Characterisation - Considering Key Questions
European BioPharmaceutical Review, 64 - 67, 2003

Dell A, Chalabi S, Easton RL, Haslam SM, Sutton-Smith M, Patankar MS, Lattanzio F, Panico M, Morris HR, Clark GF.
Murine and human zona pellucida 3 derived from mouse eggs express identical O-glycans.
Proc Natl Acad Sci 100:15631-6.(2003)

Moody AM, North SJ, Reinhold B, Van Dyken SJ, Rogers ME, Panico M, Dell A, Morris HR, Marth JD & Reinherz EL.
Sialic Acid Capping of CD8beta Core 1-O-Glycans Controls Thymocyte-Major Histocompatability Complex Class I Interaction.
Journal of Biological Chemistry 278, 7240-7246 (2003)

Michell SL, Whelan AO, Wheeler PR, Panico M, Easton RL, Etienne A, Haslam SM, Dell A, Morris HR, Reason AJ, Herrmann L.J, Young DB and Hewinson GR.
The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1,3) mannobiose moieties.
Journal of Biological Chemistry 278, 16423-16432 (2003)

Lucchiari-Hartz M, Lindo V, Hitziger N, Gaedicke S, Saveanu L, van Endert PM, Greer F, Eichmann K, Niedermann G.
Differential proteasomal processing of hydrophobic and hydrophilic protein regions: contribution to cytotoxic T lymphocyte epitope clustering in HIV-1-Nef.
Proc Natl Acad Sci U S A. 100(13):7755-60 (2003)

Wong NK, Easton RL, Panico M, Sutton-Smith M, Morrison JC, Lattanzio FA, Morris HR, Clark GF, Dell A, Patankar MS.
Characterization of the oligosaccharides associated with the human ovarian tumor marker CA125
Journal of Biological Chemistry 278, 28619-34 (2003)

Greer F, Reason AJ & Rogers M.
Post-Translational Modifications of Biopharmaceuticals – a Challenge for Analytical Characterisation.
European BioPharmaceutical Review, 106-111, Summer 2002

Reason AJ.
Validation of Amino Acid Analysis Methods
Methods in Molecular Biology 211, Protein Sequencing Protocols, 2nd ed. Edited by: BJ Smith, Humana Press Inc, Totowa, NJ, 181-194 (2002)

Hughes MJG, Moore JC, Lane JD, Wilson R, Pribul PK, Younes ZN, Dobson RJ, Everest P, Reason AJ, Redfern JM, Greer FM, Paxton T, Panico M, Morris HR, Feldman RG and Santangelo JD.
Identification of Major Outer Surface Proteins of Streprococcus agalactiae
Infection and Immunity 70, 1254-1259 (2002)

Greer F & Morris HR.
Electrospray Mass Spectrometry of Peptides, Proteins and Glycoproteins.
Methods in Molecular Biology 211, Protein Sequencing Protocols, 2nd ed. Edited by: BJ Smith, Humana Press Inc, Totowa, NJ, 195-210 (2002)

Prior JL, Parkhill J, Hitchen PG, Mungall KL, Stevens K, Morris HR, Reason AJ, Oyston PC, Dell A, Wren BW Titball RW.
The failure of different strains of Yersinia pestis to produce lipopolysaccharide O-antigen under different growth conditions is due to mutations in the O-antigen gene cluster.
FEMS Microbiol Letters 197(2), 229-233 (2001)

Greer FM
MS analysis of biopharmaceutical products
Innovations in Pharmaceutical Technology 7, 83-90 (2001)

Dell A, Morris HR
Glycoprotein structure determination by mass spectrometry
Science 291(5512), 2351-2356 (2001) Review

Prior JL, Hitchen PG, Williamson ED, Reason AJ, Morris HR, Dell A, Wren BW & Titball RW.
Characterization of the Lipopolysaccharide of Yersinia pestis.
Microbial Pathogenesis 30, 49-57 (2001).

Quin SJ, Seixas EM, Cross CA, Berg M, Lindo V, Stockinger B, Langhorne J.
Low CD4(+) T cell responses to the C-terminal region of the malaria merozoite surface protein-1 may be attributed to processing within distinct MHC class II pathways.
Eur J Immunol. 31(1):72-81 (2001)

Otvos L Jr, O I, Rogers ME, Consolvo PJ, Condie BA, Lovas S, Bulet P, Blaszcyk-Thurin M.
Interaction between heat shock proteins and antimicrobial peptides.
Biochemistry 39(46), 14150-14159 (2000)

Egner PA, Stansbury KH, Snyder EP, Rogers ME, Hintz PA, Kensler TW.
Identification and characterisation of chlorin e(4) ethyl ester in sera of individuals participating in the chlororphyllin chemoprevention trial.
Chem Res Toxicol. 13(9), 900-906 (2000)

Otvos L Jr, Pease AM, Bokony K, Glies-Davis W, Rogers ME, Hintz PA, Hoffmann R, Ertl HC.
In situ stimulation of a T helper cell hybridoma with a cellulose-bound peptide antigen.
Journal of Immunological Methods 233(1-2), 95-105 (2000).

Scragg IG, Kwiatkowski D, Vidal V, Reason A, Paxton T, Panico M, Dell A and Morris HR.
Structural Characterization of the Inflammatory Moiety of a Variable Major Lipoprotein of Borrelia recurrentis.
Journal of Biological Chemistry 275, 937-941 (2000).

Hughes GJ, Reason AJ, Savoy L, Jaton J, Frutiger-Hughes S.
Carbohydrate moieties in human secretory component.
Biochimica Biophysica Acta, 1434(1), 86-93 (1999).

Haslam SM, Houston KM, Harnett W, Reason AJ, Morris HR & Dell A.
Structural Studies of N-Glycans of Filarial Parasites - Conservation of Phosphorylcholine-Substituted Glycans among Species and Discovery of Novel Chito-Oligomers.
Journal of Biological Chemistry 274, 20953-20960 (1999).

Otvos L Jr, Hoffmann R, Xiang ZQ, O I, Deng H, Wysocka M, Pease AM, Rogers ME, Blasczyk-Thurin M, Ertl HC.
A monoclonal antibody to a multiphosphorylated, conformational epitope at the carboxy-terminus of p53.
Biochimica Biophysica Acta 1404(3), 457-474 (1998).

Greer FM.
The furure role of mass spectrometry for "well-characterised" or specified biomolecules.
Journal of Biotechnology in Healthcare 4, 314-320 (1998).

Jean F, Stella K, Thomas L, Liu G, Xiang Y, Reason AJ, Thomas G.
alpha1-Antitrypsin Portland, a bioengineered serpin highly selective for furin: application as an antipathogenic agent
Proceedings of the National Academy of Science U S A 95(13), 7293-7298 (1998).

Haslam SM, Coles GC, Reason AJ, Morris HR & Dell A.
The Novel Core Fucosylation of Haemonchus contortus N-Glycans is Stage Specific.
Molecular and Biochemical Parasitology 93, 143-147 (1998).

Lack AG, Reason AJ, Nuckolls C, Kudchodkar BJ, Nair MP, Sundarrajan G, Pritchard PH, Morris HR & Dell A.
Characterization of Recombinant human plasma lecithin: cholesterol acyltransferase (LCAT): N-linked carbohydrate structures and catalytic properties.
Journal of Lipid Research 39, 807-820 (1998).

Shaw NM, Birch OM, Tinschert A, Venetz V, Dietrich R, Savoy LA.
Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichometry of S-adenosylmethoinine use.
Biochem J. 330, 1079-1085 (1998).

Billker O, Lindo V, Panico M, Etienne AE, Paxton T, Dell A, Rogers M, Sinden RE & Morris HR. Identification of Xanthurenic Acid as the Putative Inducer of Malaria Development in the Mosquito.
Nature 392, 289-292 (1998).

Chizhov AO, Dell A, Morris HR, Reason AJ, Haslam SM, McDowell RA, Chizhov OS & Usov AI.
Structural Analysis of Laminarans by MALDI and FAB Mass Spectrometry.
Carbohydrate Research 310, 203-210 (1998).

Morris HR, Paxton T, Panico M, McDowell R, Dell A.
A novel geometry mass spectrometer, the Q-TOF, for low-femtomole/attomole-range biopolymer sequencing.
Journal of Protein Chemistry 16(5), 469-479 (1997).

Hughes GJ, Frutiger S, Savoy LA, Reason AJ, Morris HR, Jaton JC.
Human free secretory component is composed of the first 585 amino acids residue of the polymeric immunoglobilin receptor.
FEBS Letters 410(2-3), 443-446 (1997).

Miller BT, Collins TJ, Rogers ME, Kurosky A.
Peptide biotinylation with amine-reactive esters: differential side chain reactivity.
Peptides 18(10), 1585-1595 (1997).