Analytical Ultracentrifugation (AUC)
Analytical Ultracentrifugation (AUC) is used to analyse biological molecules in specially designed centrifuges, which can separate a biological molecule into its multimeric states. The sedimentation rate of the molecule is monitored continuously to produce a molecular weight profile of the species observed and, hence, the multimeric state(s) of that molecule, including determining whether aggregation is present. Equilibrium analysis can also be performed, to provide dynamic equilibrium information i.e. association/dissociation rates of the multimeric states.
AUC is carried out on SGS M-Scan’s Beckman-Coulter XL-A Analytical Ultracentrifuge.
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is performed to obtain sedimentation coefficient values and the relative percentages of monomer/multimer components and aggregates observed in supplied samples.
Sedimentation equilibrium analytical ultracentrifugation (SE-AUC) can also be undertaken if required, to assess interaction and dissociation between sample components.
AUC and SEC-MALS provide a reliable set of orthogonal data to allow an assessment of aggregation state of a biopolymer product in solution.