Capillary Electrophoresis (CE)
Capillary IsoElectric Focusing (cIEF) delivers superior resolution and reproducibility, compared to conventional Capillary Electrophoresis (CE). cIEF is free solution IsoElectric Focusing (IEF) in a capillary column. The iCE280 analyser at SGS M-Scan detects focused protein zones using a whole column UV absorption detector that avoids disturbing these focused zones. This technology is unique in that it has the comparable resolution of traditional gel IEF, but incorporates the advantages of a column-based separation technology, including quantitation (using UV at 280nm) and automation.
The iCE280 analyser is superior to conventional CE performed with single point detection systems, which require a lengthy mobilisation phase, which can potentially lead to sample degradation during the experiment. As a result, method development and sample analysis can be run on the iCE 280 instrument in a fraction of the time required by other technologies.
cIEF is used to determine the isoelectric point of a protein and to establish the presence of charge heterogeneity, due to post-translational modifications, such as glycosylation or deamidation.
Isoelectric focusing and Capillary Electrophoresis (CE) are key parts of the ICH Q6B guidelines for characterisation and confirmation of biopharmaceuticals in support of new marketing applications.